Журнал молекулярной биологии и методов

Gel electrophoresis

Gel electrophoresis is a methodology for separating and analyzing macromolecules (DNA, RNA and proteins) and their fragments on their size and charge.This technique primarily influences in clinical chemistry to separate proteins by charge or size, in organic chemistry and biology to separate a mixed population of deoxy-ribonucleic acid and ribonucleic acid fragments by length, to estimate the size of DNA & RNA fragments or to separate proteins by charge. It uses a gel as an anti-convective medium electrophoresis, the movement of a charged particle in an electrical field by which gels suppress the thermal convection caused by electric field, and as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the completed partition, so that a post electrophoresis stain can be applied.