Журнал прикладной биоинформатики и вычислительной биологии

Deficiencies in Repair of Double–Standard DNA/RNA- Binding Molecules Identified in Many Types of Solid and Liquid Tumors Oncology in Human Body for Advancing Cancer Immunotherapy Using Computer Simulations and Data Analysis

Alireza Heidari

We have recently discovered that deficiencies in repair of double– standard DNA/RNA–binding molecules identified in many types of solid and liquid tumors oncology in human body for advancing cancer immunotherapy at their upper rims bind tightly to double– standard DNA/RNA–binding molecules, irrespective of their number of base–pairs. For this aim, we have synthesized a various Fullerene Nano molecules, which have central C20, C60, C240, C540, C960, C2160 and C3840 Carbon chains [1-7]. In spite of their increasing lipophilicity, these compounds could all be dissolved completely in aqueous HEPES (4–(2–hydroxyethyl)–1–piperazineethanesulfonic acid) buffer with 50% added methanol. The binding constants among double–standard DNA/RNA–binding molecules and the above mentioned various Fullerene Nano molecules, were in the same range. That means the length of the spacer have not a great influence on the binding site. In this editorial, we have to change the spacer to the nucleic acids derivatives, which bind probably in the major groove of double–standard DNA/ RNA–binding molecules. The new various Fullerene Nano molecules were characterized by 1HNMR, 13CNMR, 31PNMR, Attenuated Total Reflectance Fourier Transform Infrared (ATR–FTIR), FT–Raman, UV– Vis and HR Mass spectroscopies. After the synthesis of the first Fullerene Nano molecule, we have found that in this type of various Fullerene Nano molecules, the binding constant between double–standard DNA/RNA– binding molecules group (a) and double–standard DNA/RNA–binding molecules group (b) was greater than the latter.