Архивы медицинской биотехнологии

Evaluation of an Intragastric Model for Clinically Isolated Pathogenic Shigella Species-Induced Diarrhea in Albino Wistar Rats

Ashajyothi C* , Prabhurajeshwar C , Navya HM, and Kelmani Chandrakanth R

Shigella is a main reason of dysentery all through the arena and is liable for 5-10 percentage of diarrheal contamination in many areas and also constitutes one of the developing numbers of antimicrobial-resistance micro-organism in growing countries. Diarrhea is a condition where there is stomach pain, fever, vomiting and excessive passage of very watery stools in initial stages are common. However, in serious cases, there can be passage of blood and mucus-streaked stools as well. For the pre-clinical evaluation of pathogenesis, screening the therapeutics and evaluating vaccines, wistar rats and different primates are the handiest animals which can be evidently vulnerable to shigellosis. In this study, wistar rats were divided into various groups and through oral and intragastrical route of administration, a dose of Shigella strains (S. dysentriae-07 and S. flexneri-277) were injected with varying numbers of Shigella strain. Based on better survival rate and real induction of diarrhea, intragastric administration was considered the most effective with a dose of 12 × 108 cfu/ml of Shigella strains. Further, diarrheal induction was associated with abdominal ailment; progressive increase in stool weight and increase in bacterial populations were observed. This study concludes that the Shigella induced rat model of shigellosis which might be helpful for physiopathological, pharmaceutical studies, for evaluation of vaccine and test the efficacy of microbial agents.